In vitro investigations of biological activities of Thymus willdenowii and Thymus atlanticus polyphenol-rich extracts

Thyme species produce a wide variety of phenolic compounds including tannins, acids, and flavonoids. Thymus atlanticus (T. atlanticus) willdenowii willdenowii) are important thyme in the southeast Morocco, with numerous biological properties. The polyphenolic extracts these two were obtained using ethanol through Soxhlet apparatus. Antioxidant (DPPH, FRAP, TAC methods), antihemolytic (2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) induced hemolysis test), hypolipidemic (3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity inhibition anti-inflammatory (protein denaturation inhibition) effects carried out vitro methods. results showed that revealed amounts compounds. contents flavonoids significant species, while tannins anthocyanin very low. T. an antioxidant considerable effect AAPH-induced test (IC50 = 0.29 mg/mL), heat-induced protein 1.61 mg/mL). Moreover, both at dose 20 µg/mL by inhibiting HMG-CoA willdenowii: 51.16 %; atlanticus: 62.83 %). In conclusion, have antioxidant, antihemolytic, hypolipidemic, effects. richness polyphenols gives them large specter properties, making valuable source natural bioactive could prevent or treat various diseases.